Summary
The MicroStacker™ technology allows well-controlled layered stacking of F(ab’) fragments of IgG secondary antibody and peroxidase enzymes on a micro-polymer scaffold, which results in a compact polymeric structure with superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. This system avoids the use of streptavidin and biotin, and therefore eliminates non-specific staining as a result of endogenous biotin. Herein, the Mouse-on-Mouse Polymer comprises of specially engineered anti-Mouse secondary antibody to eliminate the high backgrounds caused by endogenous mouse antibodies in mouse tissues.
Polymer
Product Features
Staining Procedure:
Primary antibody incubation: RT, 30-60min
Secondary antibody incubation: RT, 15min
DAB incubation: 5min
Case study
MicroStackerTM Mouse-on-Mouse
Detection polymers in comparison:
A internationally famous brand
Staining Photos:
Mouse spleen – SMA-1%-PEG:
MicroStacker™ Mouse-on-Mouse Polymer Detection Kit
