Product Features

• 1-Step System – Simple and efficient manual workflow based on proprietary MicroStacker™ technology

• Specific Detection – Designed to detect mouse primary antibodies on human tissue with high accuracy

• Bright, High-Contrast Signal – Utilizes Fast Red substrate, providing a vivid signal with excellent contrast against background

• Rapid and Reliable – Delivers clear and sharp staining in less time compared to conventional AP polymers

Recommended Protocol

• Primary Antibody: Room temperature, 30-60 minutes

• Detection Polymer: Room temperature, 30 minutes

• Fast Red Chromogen: 6-8 minutes

Application Notes

• Ideal for Pigmented Tissues – Suitable for tissues with endogenous pigments, such as melanoma and lung cancer, ensuring clear visualization against dark backgrounds

• Multiplex Compatibility – Suitable for sequential multiplex IHC applications, allowing multiple targets to be detected on the same tissue section without cross-reactivity

MicroStacker™ Technology Platform

The MicroStacker™ Goat Anti-Mouse AP Polymer is a biotin-free, alkaline phosphatase (AP) polymer system designed for detecting mouse primary antibodies on human tissue. This advanced system incorporates Fast Red chromogen to produce high-contrast staining, even in pigmented tissues.

Celnovte’s proprietary MicroStacker™ technology employs micro-polymer scaffolds that enable directional attachment of Fab’ fragments to the poly-AP core. This design prevents antibody binding sites from being blocked during conjugation, resulting in enhanced sensitivity and minimal background interference.

The compact polymeric structure allows for deep penetration into tissue, providing uniform staining across nuclear, cytoplasmic, and membranous antigens. By eliminating endogenous biotin interference and non-specific Fc receptor binding, the system ensures exceptional specificity and reproducibility, making it ideal for challenging immunohistochemical applications and sequential multiplex IHC workflows.