About
The Alcian Blue - PAS Stain Kit is intended for use in the simultaneous histological visualization of sulfated and carboxylated acid mucopolysaccharides, sulfated and carboxylated sialomucins and neutral mucins.
Alcian Blue, a copper phthalocyanine dye, binds acid mucosubstances. When used in a pH 2.5 acid solution Alcian Blue stains sulfated and carboxylated acid mucosubstances. Neutral mucins are stained with a standard Periodic Acid Schiff (PAS) reaction. Neutral mucins are oxidized by Periodic Acid forming free aldehydes capable of binding Schiff’s Solution resulting in a magenta end product. Hematoxylin is provided as a nuclear counterstain.
Specification
| Components | 1 kit |
|---|---|
| Acetic Acid Solution (3%) | 2 x 125ml |
| Alcian Blue Solution, pH 2.5 | 1 x 250ml |
| Hematoxylin (Modified Mayer's Solution) | 1 x 100ml |
| Periodic acid solution | 1 x 100ml |
| Schiff's solution | 1 x 100ml |
Product Advantage
- Great Stability
- Bright Stain Color
Expected Results
- Acidic Sulfated Mucosubstances: Blue
- Hyaluronic Acid: Blue
- Sialomucins: Blue
- Neutral Mucins: Magenta
- Mixtures of Acidic and Neutral Mucins: Blue - Mauve depending on dominant entity
Controls Tissue
Small Intestine, Colon
Storage / Shelf Life
2-8 ℃ / 18 months
The Alcian Blue - PAS Stain Kit is intended for use in the simultaneous histological visualization of sulfated and carboxylated acid mucopolysaccharides, sulfated and carboxylated sialomucins and neutral mucins.
Alcian Blue, a copper phthalocyanine dye, binds acid mucosubstances. When used in a pH 2.5 acid solution Alcian Blue stains sulfated and carboxylated acid mucosubstances. Neutral mucins are stained with a standard Periodic Acid Schiff (PAS) reaction. Neutral mucins are oxidized by Periodic Acid forming free aldehydes capable of binding Schiff’s Solution resulting in a magenta end product. Hematoxylin is provided as a nuclear counterstain.
Specification
| Components | 1 kit |
|---|---|
| Acetic Acid Solution (3%) | 2 x 125ml |
| Alcian Blue Solution, pH 2.5 | 1 x 250ml |
| Hematoxylin (Modified Mayer's Solution) | 1 x 100ml |
| Periodic acid solution | 1 x 100ml |
| Schiff's solution | 1 x 100ml |
Product Advantage
- Great Stability
- Bright Stain Color
Expected Results
- Acidic Sulfated Mucosubstances: Blue
- Hyaluronic Acid: Blue
- Sialomucins: Blue
- Neutral Mucins: Magenta
- Mixtures of Acidic and Neutral Mucins: Blue - Mauve depending on dominant entity
Controls Tissue
Small Intestine, Colon
Storage / Shelf Life
2-8 ℃ / 18 months
Alcian Blue - PAS Stain Kit
- Regular price
- $299.00
- Sale price
- $299.00
- Regular price
-
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