The TSA Fluorophore CM480 offers green fluorescence in multiplex immunohistochemistry (mIHC) applications, utilizing the Tyramide Signal Amplification (TSA) technique. This technique relies on horseradish peroxidase (HRP)-labeled secondary antibodies to catalyze the deposition of the CM480 fluorophore onto tyrosine residues in the antigen. The covalent binding of the fluorophore ensures highly specific visualization of the target antigen, enhancing the sensitivity of detection and allowing for the clear identification of green-fluorescing biomarkers.